Deguelin exhibits antiproliferative activity against various cancer cell types. Previous studies have reported that deguelin exhibits pro-apoptotic activity against human cancer cells. The current study aimed at further elaborating the anticancer effects of deguelin against multiple myeloma cells. Cell growth estimations were made through MTT assay. Phase contrast microscopy was used for the analysis of the viability of multiple myeloma cells. Colony formation from multiple myeloma cells was studied using a clonogenic assay. Antioxidative assays for determining levels of glutathione (GSH) and superoxide dismutase (SOD) were carried out after treating multiple myeloma cells with deguelin. The apoptosis of multiple myeloma cells was studied using AO/EB and Annexin V-FITC/PI staining methods. Multiple myeloma cell cycle analysis was performed through flow cytometry. mRNA expression levels were depicted using qRT-PCR. Migration and invasion of multiple myeloma cells were determined with the wound-healing and transwell assays, respectively. Deguelin specifically inhibited the multiple myeloma cell growth while the normal plasma cells were minimally affected. Multiple myeloma cells when treated with deguelin exhibited remarkably lower viability and colony-forming ability. Multiple myeloma cells treated with deguelin produced more SOD and had higher GSH levels. The multiple myeloma cell growth, migration, and invasion were significantly declined by in vitro administration of deguelin. In conclusion, deguelin treatment, when applied in vitro, induced apoptotic cell death and resulted in mitotic cessation at the G2/M phase through modulation of cell cycle regulatory mRNAs in multiple myeloma cells.
Multiple Myeloma , Proto-Oncogene Proteins c-akt , Rotenone/analogs & derivatives , Humans , Proto-Oncogene Proteins c-akt/metabolism , Signal Transduction , Multiple Myeloma/drug therapy , Cell Line, Tumor , Cell Cycle Checkpoints , Apoptosis , Cell Proliferation , Superoxide Dismutase/metabolism , Superoxide Dismutase/pharmacology , p38 Mitogen-Activated Protein Kinases/metabolism
Subnanometer pores/channels (SNPCs) play crucial roles in regulating electrochemical redox reactions for rechargeable batteries. The delicately designed and tailored porous structure of SNPCs not only provides ample space for ion storage but also facilitates efficient ion diffusion within the electrodes in batteries, which can greatly improve the electrochemical performance. However, due to current technological limitations, it is challenging to synthesize and control the quality, storage, and transport of nanopores at the subnanometer scale, as well as to understand the relationship between SNPCs and performances. In this review, we systematically classify and summarize materials with SNPCs from a structural perspective, dividing them into one-dimensional (1D) SNPCs, two-dimensional (2D) SNPCs, and three-dimensional (3D) SNPCs. We also unveil the unique physicochemical properties of SNPCs and analyse electrochemical couplings in SNPCs for rechargeable batteries, including cathodes, anodes, electrolytes, and functional materials. Finally, we discuss the challenges that SNPCs may face in electrochemical reactions in batteries and propose future research directions.
As the demand for ethylene grows continuously in industry, conversion of ethane to ethylene has become more and more important; however, it still faces fundamental challenges of low ethane conversion, low ethylene selectivity, overoxidation, and instability of catalysts. Electrooxidative dehydrogenation of ethane (EODHE) in a solid oxide electrolysis cell (SOEC) is an alternative process. Here, a multiphase oxide Ce0.6Mn0.3Fe0.1O2-δ-NiFe-MnOx has been fabricated by a self-assembly process and utilized as the SOEC anode material for EODHE. The highest ethane conversions reached 52.23% with 94.11% ethylene selectivity at the anode side and CO with 10.9 mL min-1 cm-2 at the cathode side, at 1.8 V at 700 °C. The remarkable electrooxidative performance of CMF-NiFe-MnOx is ascribed to the NiFe alloy and MnOx nanoparticles and improvement of the concentration of oxygen vacancies within the fluorite substrate, generating dual active sites for C2H6 adsorption, dehydrogenation, and selective transformation of hydrogen without overoxidizing the ethylene generated. Such a tailored strategy achieves no significant degradation observed after 120 h of operation and constitutes a promising basis for EODHE.
The low-cost transition metal oxides have drawn widespread interest as alternatives to noble metal-based electrocatalysts for oxygen evolution reaction (OER). Transition metal oxides usually undergo surface reconstruction during electrochemical reaction to form the actual active species. However, in-depth understanding and regulating of the surface reconstruction of active phases for oxides in OER remains an onerous challenge. Herein, we report a simple Fe element substitution strategy to facilitate the surface reconstruction of spinel oxide NiCr2O4 to generate active (oxy)hydroxides. The activated Fe-doped NiCr2O4 (Act-Fe-NCO) exhibits a lower OER overpotential of 259 mV at 10 mA cm-2 than activated NiCr2O4 (Act-NCO, 428 mV), and shows excellent stability for 120 h. The electrochemically activated CV measurement and nanostructure characterizations reveal that Fe substitution could promote the consumption of lattice oxygen during electrochemical activation to induce the leaching of soluble Cr cations, thereby facilitating the reconstruction of remaining Ni cations on the surface into (oxy)hydroxide active species. Moreover, theoretical calculations further demonstrate that the O 2p band center of NiCr2O4 moves towards the Fermi level due to Fe substitution, thus promoting lattice oxygen oxidation and providing greater structural flexibility for surface reconstruction. This work shows a promising way to regulate the surface reconstruction kinetics and OER electrocatalytic activity of transition metal oxides.
Acute lung injury (ALI) is an acute infectious diseases caused by a variety of factors. The function of TTC4 in sepsis-induced lung injury remains largely unknown. This study aimed to explore the critical role of TTC4 in sepsis-induced lung injury. Mice anaesthetized using pentobarbital sodium and subjected to cecal ligation and puncture (CLP) surgery. TTC4 expression levels in patients with sepsis-induced lung injury were down-regulated. The inhibition of TTC4 gene promoted lung injury in mice model of sepsis. TTC4 gene improved inflammation in vitro model and mice model. TTC4 gene reduced pyroptosis in macrophages of sepsis-induced lung injury by the inhibition of mitochondrial damage. TTC4 gene induced HSP70 expression to reduce NLRP3-induced pyroptosis in macrophages. TTC4 protein interlinked HSP70 protein. The activation of HSP70 reduced the effects of sh-TTC4 in model of sepsis-induced lung injury through mitochondrial damage. m6A-forming enzyme METTL3 reduced TTC4 stability. Our study suggests the m6A forming enzyme METTL3 control TTC4 reduced inflammation and pyroptosis in model of sepsis-induced lung injury through inhibition of mitochondrial damage by HSP70/ROS/NLRP3 signaling pathway, TTC4 gene as an represents a potential therapeutic strategy for the treatment of sepsis-induced lung injury.
Lithium (Li) dendrite growth in a routine carbonate electrolyte (RCE) is the main culprit hindering the practical application of Li metal anodes. Herein, we realize the regulation of the LiPF6 decomposition pathway in RCE containing 1.0 M LiPF6 by introducing a "self-polymerizing" additive, ethyl isothiocyanate (EITC), resulting in a robust LiF-rich solid electrolyte interphase (SEI). The effect of 1 vol % EITC on the electrode/electrolyte interfacial chemistry slows the formation of the byproduct LixPOFy. Such a LiF-rich SEI with EITC polymer winding exhibits a high Young's modulus and a uniform Li-ion flux, which suppresses dendrite growth and interface fluctuation. The EITC-based Li metal cell using a Li4Ti5O12 cathode delivers a capacity retention of 81.4% over 1000 cycles at 10 C, outperforming its counterpart. The cycling stability of 1 Ah pouch cells was further evaluated under EITC. We believe that this work provides a new method for tuning the interfacial chemistry of Li metal through electrolyte additives.
Background: Upper tract urothelial carcinoma (UTUC) is a relatively rare disease with a poor prognosis. A growing body of evidence demonstrates that inflammation and the inflammatory microenvironment play a crucial role in tumorigenesis and tumor progression. Our aim was to evaluate the prognostic value of blood inflammation markers and develop a prediction model that incorporates inflammation markers in order to predict overall survival (OS) of UTUC. Methods: We included 304 localized UTUC patients from two medical institutions who had undergone radical nephroureterectomy (RNU) (167 in the training cohort, 137 in the validation cohort). Univariate and multivariate Cox regression analyses were performed to screen the prognostic factors, and a nomogram and a web-based calculator were generated based on these predictors. The Harrell's concordance index (C-index), the area under the receiver operating characteristic (ROC) curve, the calibration curve, and decision curve analysis (DCA) were used to evaluate the performance of the nomogram. Results: Independent predictors incorporated in the nomogram were pathological stage, surgical margin, albumin-to-globulin ratio (AGR), and hemoglobin-to-red cell distribution width ratio (HRR). The c-index value was 0.726 in the training cohort and 0.761 in the validation cohort. The area under the ROC of the nomogram at 1-, 3- and 5-year in the training and validation sets were 0.765, 0.755, 0.763, and 0.791, 0.833, 0.802, respectively. Both the internal and external validation calibration plots showed a subtle distinction between the predicted and the actual probabilities. And it appears to provide incremental benefits for clinical decision-making in comparison to the American Joint Committee of Cancer (AJCC) staging system. Conclusions: In patients with UTUC after RNU, lower preoperative AGR and HRR were independent predictors of inferior survival. In addition, we created a novel blood inflammation marker-based dynamic nomogram that may be useful for surgeons or oncologists in risk stratification and patient selection for more intensive therapy and closer follow-up.
The graphite/lithium metal hybrid anode shows great potential for achieving high-specific-energy lithium batteries. Despite the "dead lithium" problem caused by repeated stripping and deposition of Li component based on a conversion reaction, the degradation mechanism, based on intercalation reaction, of graphite in a hybrid anode is generally ignored. In this contribution, through in situ X-ray diffraction and in situ Raman analysis, we reveal that hysteresis and the mixed-phase state of graphite during deintercalation play a critical role in hybrid battery degradation. On the other hand, we successfully mitigated graphite degradation and increased the reversible capacity of the hybrid anode by introducing an inorganic-rich solid electrolyte interface. Remarkably, the hybrid anode (30% higher specific capacity compared to graphite) exhibits an average coulombic efficiency of 99.11% and retains 96.13% of initial capacity over 120 cycles. This work sheds new light on the advancement of high-specific-energy lithium secondary batteries.
Lithium-sulfur (Li-S) batteries are widely studied because of their high theoretical specific capacity and environmental friendliness. However, the further development of Li-S batteries is hindered by the shuttle effect of lithium polysulfides (LiPSs) and the sluggish redox kinetics. Since the adsorption and catalytic conversion of LiPSs mainly occur on the surface of the electrocatalyst, regulating the surface structure of electrocatalysts is an advisable strategy to solve the obstacles in Li-S batteries. Herein, CoP nanoparticles with high oxygen content on surface embedded in hollow carbon nanocages (C/O-CoP) is employed to functionalize the separators and the effect of the surface oxygen content of CoP on the electrochemical performance is systematically explored. Increasing the oxygen content on CoP surface can enhance the chemical adsorption to lithium polysulfides and accelerate the redox conversions kinetics of polysulfides. The cell with C/O-CoP modified separator can achieve the capacity of 1033 mAh g-1 and maintain 749 mAh g-1 after 200 cycles at 2 C. Moreover, DFT calculations are used to reveal the enhancement mechanism of oxygen content on surface of CoP in Li-S chemistry. This work offers a new insight into developing high-performance Li-S batteries from the perspective of surface engineering.
Transition metal oxides have been extensively investigated for oxygen evolution reaction (OER). While the introduction of oxygen vacancies (Vo) was found to be an effective way to enhance the electrical conductivity and the OER electrocatalytic activity of transition metal oxides, the oxygen vacancies are easily damaged during the long-term catalytic process, resulting in rapid decay of the electrocatalytic activity. Herein, we proposed the strategy of dual-defect engineering to enhance the catalytic activity and stability of NiFe2O4 by filling the oxygen vacancies of NiFe2O4 with phosphorus atoms. The filled P atoms could form coordination with iron and nickel ions to compensate the coordination number and optimize the local electronic structure, which not only enhances the electrical conductivity but also improves the intrinsic activity of the electrocatalyst. Meanwhile, the filling of P atoms could stabilize the Vo and thus improving the cycling stability of the material. The theoretical calculation further demonstrates that the improvement in conductivity and intermediate binding by P refilling remarkably contributes to enhancing the OER activity of NiFe2O4-Vo-P. Benefiting from the synergistic effect of filled P atoms and Vo, the derived NiFe2O4-Vo-P exhibits fascinating activity with ultra-low OER overpotentials of 234 and 306 mV at 10 and 200 mA cm-2, together with the good durability for 120 h at relatively high current density of 100 mA cm-2. This work sheds light on the design of high-performance transition metal oxide catalysts through defect regulation in the future.
The electrocatalytic conversion of polysulfides is crucial to lithium-sulfur batteries and mainly occurs at triple-phase interfaces (TPIs). However, the poor electrical conductivity of conventional transition metal oxides results in limited TPIs and inferior electrocatalytic performance. Herein, a TPI engineering approach comprising superior electrically conductive layered double perovskite PrBaCo2O5+δ (PBCO) is proposed as an electrocatalyst to boost the conversion of polysulfides. PBCO has superior electrical conductivity and enriched oxygen vacancies, effectively expanding the TPI to its entire surface. DFT calculation and in situ Raman spectroscopy manifest the electrocatalytic effect of PBCO, proving the critical role of enhanced electrical conductivity of this electrocatalyst. PBCO-based Li-S batteries exhibit an impressive reversible capacity of 612 mAh g-1 after 500 cycles at 1.0 C with a capacity fading rate of 0.067% per cycle. This work reveals the mechanism of the enriched TPI approach and provides novel insight into designing new catalysts for high-performance Li-S batteries.
For developing the reversible lithium metal anode, constructing an ideal solid electrolyte interphase (SEI) by regulating the Li+ solvation structure is a powerful way to overcome the major obstacles of lithium dendrite and limited Coulombic efficiency (CE). Herein, spherical mesoporous molecular sieve MCM-41 nanoparticles are coated on a commercial PP separator and used to regulate the Li+ solvation structure for lithium metal batteries (LMBs). The regulated solvation structure exhibits an agminated state with more contact ion pairs (CIPs) and ionic aggregates (AGGs), which successfully construct a homogeneous inorganic-rich SEI in the lithium anode. Meanwhile, the regulated solvation structure weakens the interaction between the solvents and Li+, resulting in low Li+ desolvation energy and uniform Li deposition. Thus, a high CE (â¼96.76%), dendrite-free Li anode, and stable Li plating/stripping cycling for approximately 1000 h are achieved in the regulated carbonate-based electrolyte without any additives. Therefore, regulating the Li+ solvation structure in the electrolyte by employing a mesoporous material is a forceful way to construct an ideal SEI and harness lithium metal.
Carbon dioxide (CO2) gas is the main cause of global warming and has a significant effect on both climate change and human health. In this study, Ni/Ti co-doped Sr1.95Fe1.2Ni0.1Ti0.2Mo0.5O6-δ (SFNTM) double perovskite oxides were prepared and used as solid oxide electrolysis cell (SOEC) cathode materials for effective CO2 reduction. Ti-doping enhances the structural stability of the cathode material and increases the oxygen vacancy concentration. After treatment in 10% H2/Ar at 800°C, Ni nanoparticles were exsolved in situ on the SFNTM surface (Ni@SFNTM), thereby improving its chemisorption and activation capacity for CO2. Modified by the Ti-doping and the in situ exsolved Ni nanoparticles, the single cell with Ni@SFNMT cathode exhibits improved catalytic activity for CO2 reduction, exhibiting a current density of 2.54 A cm-2 at 1.8 V and 800°C. Furthermore, the single cell shows excellent stability after 100 h at 1.4 V, indicating that Ni/Ti co-doping is an effective strategy for designing novel cathode material with high electrochemical performance for SOEC.
Defective materials have been demonstrated to possess adsorptive and catalytic properties in lithium-sulfur (Li-S) batteries, which can effectively solve the problems of lithium polysulfides (LiPSs) shuttle and sluggish conversion kinetics during charging and discharging of Li-S batteries. However, there is still a lack of research on the quantitative relationship between the defect concentration and the adsorptive-catalytic performance of the electrode. In this work, perovskites Sr0.9 Ti1- x Mnx O3- δ (STMnx ) (x = 0.1-0.3) with different oxygen-vacancy concentrations are quantitatively regulated as research models. Through a series of tests of the adsorptive property and electrochemical performance, a quantitative relationship between oxygen-vacancy concentration and adsorptive-catalytic properties is established. Furthermore, the catalytic mechanism of oxygen vacancies in Li-S batteries is investigated using density functional theory calculations and in situ experiments. The increased oxygen vacancies can effectively increase the binding energy between perovskite and LiPSs, reduce the energy barrier of LiPSs decomposition reaction, and promote LiPSs conversion reaction kinetics. Therefore, the perovskite STMn0.3 with high oxygen-vacancy concentrations exhibits excellent LiPSs adsorptive and catalytic properties, realizing high-efficiency Li-S batteries. This work is helpful to realize the application of the quantitative regulation strategy of defect engineering in Li-S batteries.
Bladder cancer is the second-most common malignancy in the urogenital system and the most common in men. However, our understanding of the driving mechanisms of bladder cancer remains incomplete. The forkhead box (FOX) family of transcription factors is implicated in urogenital development and bladder malignancies. Many exosomal microRNAs have been identified as regulators and mediators of the expression of FOX, including the expression of FOXC1. miR-4792 has been known as a tumor miRNA suppressor. However, the function of miR-4792/FOXC1 signaling in bladder cancer development remains unknown. Here, we studied the role of miR-4792/FOXC1 signaling in bladder cancer by using multiple bladder cancer cell lines and bladder cancer mouse models through in vitro and in vivo approaches. We showed that FOXC1 is highly expressed in multiple bladder cancer cell lines and bladder tumor tissues. The knockdown of FOXC1 expression in bladder cancer cell lines decreases c-Myc expression levels, retards cell growth, and reduces aerobic glycolysis (also known as the Warburg effect) and lactic acid content. By contrast, the overexpression of FOXC1 elicits the opposite effects. FOXC1-downregulated bladder cancer cells form significantly smaller tumors in vivo. The inhibition of c-Myc reverses the effects of FOXC1 overexpression and leads to reduced cell proliferation, aerobic glycolysis, and lactic acid content. miR-4792 expression is downregulated in bladder tumor tissues. miR-4792 exposure to bladder cancer cells reduces the expression levels of FOXC1 and c-Myc, slows down cell growth, and decreases aerobic glycolysis and lactic acid content. However, the enhanced miR-4792 expression elicits opposite effects. These findings provided the first evidence that the exosome-mediated delivery of miR-4792 could play an important role in bladder cancer development through the downregulation of FOXC1 and c-Myc, which further inhibited aerobic glycolysis and lactic acid content.
BACKGROUND: To determine the prostate cancer biochemical recurrence-related fusion biopsy characteristics before radical surgery and to establish the risk prediction model of biochemical recurrence of prostate cancer. METHODS: Three hundred and four patients undergoing radical surgery for prostate cancer at Huadong Hospital affiliated to Fudan University between 2009 and 2020 for preoperative magnetic resonance imaging (MRI) before biopsy with suspicious prostate cancer lesions. Each case was followed by a 10 + x needle combination of targeted biopsy (intentional or robotic fusion) with systematic biopsy. Prostate-specific antigen levels were measured at 1, 3, and 6 months postoperatively, followed by reexamination every 6 months. Survival analysis was performed by the Kaplan-Meier method, univariate and multivariate analysis by Cox, and Logistic risk regression models. RESULTS: Higher Prostate Imaging Reporting And Data System (PI-RADS) scores (p < 0.001), suspicious extracapsular invasion (p < 0.001), and seminal vesicle invasion (p < 0.001) on MRI, the largest lesion diameter on MRI (p = 0.006), higher biopsy International Society of Urological Pathology (ISUP) grade group (p < 0.001) related to higher biochemical recurrence rates, higher pathological staging (p < 0.001), and a greater probability of local lymph node metastasis (p < 0.001). We accurately predicted the biochemical recurrence of prostate cancer after radical surgery based on preoperative features including the long diameter of the largest MRI lesion more than 23 mm, seminal vesicle invasion on MRI, and targeted fusion biopsy ISUP grade >3 Risk stratified classification (AUC = 0.729, p < 0.001). In our cohort, this risk stratification had a larger area under the curve than predictive models based only on magnetic resonance parameters and traditional risk scores. CONCLUSIONS: In this cohort, seminal vesicle invasion on MRI, the long diameter of the largest MRI lesion, and targeted fusion biopsy ISUP grade grope are significantly predictive of pathologic features and biochemical recurrence after prostate surgery. The risk stratification integrating the three parameters could better predict the biochemical recurrence than the traditional model.
Prostate , Prostatic Neoplasms , Humans , Image-Guided Biopsy/methods , Magnetic Resonance Imaging/methods , Male , Prostate/diagnostic imaging , Prostate/pathology , Prostate/surgery , Prostatectomy/methods , Prostatic Neoplasms/diagnostic imaging , Prostatic Neoplasms/surgery , Retrospective Studies , Seminal Vesicles/pathology
Background: The incidence rate of prostate cancer is increasing rapidly. This study aims to explore the gene-associated mechanism of prostate cancer biochemical recurrence (BCR) after radical prostatectomy and to construct a biochemical recurrence of prostate cancer prognostic model. Methods: The DEseq2 R package was used for the differential expression of mRNA. The ClusterProfiler R package was used to analyze the functional enrichment of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to explore related mechanisms. The Survival, Survminer, and My.stepwise R packages were used to construct the prognostic model to predict the biochemical recurrence-free probability. The RMS R package was used to draw the nomogram. For evaluating the prognostic model, the timeROC R package was used to draw the time-dependent ROC curve (receiver operating characteristic curve). Result: To investigate the association between mRNA and prostate cancer, we performed differential expression analysis on the TCGA (The Cancer Genome Atlas) database. Seven protein-coding genes (VWA5B2, ARC, SOX11, MGAM, FOXN4, PRAME, and MMP26) were picked as independent prognostic genes by regression analysis. Based on their Cox coefficient, a risk score formula was proposed. According to the risk scores, patients were divided into high- and low-risk groups based on the median score. Kaplan-Meier plot curves showed that the low-risk group had a better biochemical recurrence-free probability compared to the high-risk group. The 1-year, 3-year, and 5-year AUCs (areas under the ROC curve) of the model were 77%, 81%, and 86%, respectively. In addition, we built a nomogram based on the result of multivariate Cox regression analysis. Furthermore, we select the GSE46602 dataset as our external validation. The 1-year, 3-year, and 5-year AUCs of BCR-free probability were 83%, 82%, and 80%, respectively. Finally, the levels of seven genes showed a difference between PRAD tissues and adjacent non-tumorous tissues. Conclusions: This study shows that establishing a biochemical recurrence prediction prognostic model comprising seven protein-coding genes is an effective and precise method for predicting the progression of prostate cancer.
Reversible solid oxide cells (RSOCs) present a conceivable potential for addressing energy storage and conversion issues through realizing efficient cycles between fuels and electricity based on the reversible operation of the fuel cell (FC) mode and electrolysis cell (EC) mode. Reliable electrode materials with high electrochemical catalytic activity and sufficient durability are imperatively desired to stretch the talents of RSOCs. Herein, oxygen vacancy engineering is successfully implemented on the Fe-based layered perovskite by introducing Zr4+, which is demonstrated to greatly improve the pristine intrinsic performance, and a novel efficient and durable oxygen electrode material is synthesized. The substitution of Zr at the Fe site of PrBaFe2O5+δ (PBF) enables enlarging the lattice free volume and generating more oxygen vacancies. Simultaneously, the target material delivers more rapid oxygen surface exchange coefficients and bulk diffusion coefficients. The performance of both the FC mode and EC mode is greatly enhanced, exhibiting an FC peak power density (PPD) of 1.26 W cm-2 and an electrolysis current density of 2.21 A cm-2 of single button cells at 700 °C, respectively. The reversible operation is carried out for 70 h under representative conditions, that is, in air and 50% H2O + 50% H2 fuel. Eventually, the optimized material (PBFZr), mixed with Gd0.1Ce0.9O2, is applied as the composite oxygen electrode for the reversible tubular cell and presents excellent performance, achieving 4W and 5.8 A at 750 °C and the corresponding PPDs of 140 and 200 mW cm-2 at 700 and 750 °C, respectively. The enhanced performance verifies that PBFZr is a promising oxygen electrode material for the tubular RSOCs.
To explore the potential function of miR-9-5p in wear-particle-induced osteoclastogenesis, we examined the expression of SIRT1 and miR-9-5p in particle-induced osteolysis (PIO) mice calvariae and polyethylene (PE)-induced RAW 264.7 cells and found that SIRT1 expression was downregulated while miR-9-5p expression was upregulated in both models. We then verified that miR-9-5p targets SIRT1. miR-9-5p was found to promote PE-induced osteoclast formation from RAW 264.7 cells by tartrate-resistant acid phosphatase staining and detection of osteoclast markers, and miR-9-5p activation of the SIRT1/NF-kB signaling pathway was found in cells by detecting the expression of SIRT1/NF-kB pathway-related proteins and rescue assays. In conclusion, we found that miR-9-5p activated the SIRT1/NF-κB pathway to promote wear-particle-induced osteoclastogenesis. miR-9-5p may be a useful therapeutic target for PIO remission and treatment.
